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American Society of Microbiology ABMM Sample Questions (Q45-Q50):

NEW QUESTION # 45
A clinical microbiology laboratory is implementing whole-genome sequencing (WGS) for enhanced surveillance of Listeria monocytogenes isolates associated with foodborne outbreaks. WGS provides several advantages over traditional subtyping methods like pulsed-field gel electrophoresis (PFGE). Which of the following is the MOST significant advantage of WGS in this context?

A. Lower cost per isolate compared to PFGE.
B. Higher discriminatory power for resolving phylogenetic relationships and identifying outbreakclusters.
C. Faster turnaround time for obtaining results.
D. Easier standardization and inter-laboratory comparison of data.

Answer: B

NEW QUESTION # 46
A patient develops a severe skin infection after a puncture wound sustained while gardening. Culture of the wound exudate grows a Gram-positive, spore-forming bacillus that is anaerobic and produces a potent neurotoxin causing sustained muscle contraction. The MOST likely organism is:

A. Clostridium perfringens
B. Clostridium botulinum
C. Bacillus anthracis
D. Clostridium tetani

Answer: D

NEW QUESTION # 47
A microbiology laboratory is asked to identify the causative agent of a fungal keratitis. Microscopic examination of corneal scrapings reveals broad, aseptate hyphae with right-angle branching. The MOST likely genus is:

A. Candida
B. Mucor or Rhizopus
C. Aspergillus
D. Fusarium

Answer: B

NEW QUESTION # 48
A neonate develops a vesicular rash, fever, and seizures within the first week of life. CSF analysis reveals lymphocytic pleocytosis and elevated protein. PCR of the CSF is positive for a double-stranded DNA virus.
The MOST likely causative agent is:

A. Human parvovirus B19
B. Cytomegalovirus (CMV)
C. Herpes simplex virus (HSV)
D. Varicella-zoster virus (VZV)

Answer: C

NEW QUESTION # 49
A clinical microbiology laboratory is investigating an outbreak of vancomycin-resistantEnterococcus faecium (VRE) in a hospital. Whole-genome sequencing of isolates from different patients reveals the presence of the vanA operon on a mobile genetic element that is highly conserved across all isolates. This finding MOST strongly suggests:

A. Independent acquisition of vancomycin resistance by different E. faecium strains.
B. Clonal spread of a single VRE strain carrying the vanA operon.
C. Horizontal transfer of the vanA operon to multiple susceptible E. faecium strains.
D. Selection of vancomycin-resistant mutants within a susceptible E. faecium population due to antibiotic pressure.

Answer: B

NEW QUESTION # 50
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